Mesh Fragmentation Improves Dissociation Efficiency in Top-down Proteomics

Mesh Fragmentation Improves Dissociation Efficiency in Top-down Proteomics

Top-down proteomics is a key mass spectrometry-based know-how for complete evaluation of proteoforms. Proteoforms exhibit a number of excessive cost states and isotopic kinds in full MS scans. The dissociation habits of proteoforms in totally different cost states and subjected to totally different collision energies is extremely variable. The present broadly employed data-dependent acquisition (DDA) technique selects a slender m/z vary (akin to a single proteoform cost state) for dissociation from essentially the most ample precursors. We describe right here Mesh, a novel dissociation technique, to dissociate a number of cost states of 1 proteoform with a number of collision energies.

We present that the Mesh technique has the potential to generate fragment ions with improved sequence protection and enhance identification ratios in top-down proteomic analyses of complicated samples. The technique is carried out inside an open-source instrument management software program program named MetaDrive to carry out actual time deconvolution and precursor choice. To higher perceive molecular mechanisms and discover biomarkers for the pathogen throughout LTBI, we cultured strains of Mycobacterium tuberculosis (Mtb) beneath stress situations, mimicking these in the host granuloma intracellular setting, to induce entry into the non-replicating persistence stage.

The stresses included hypoxia, low pH (5.0), iron deprivation (100 μM of two, 2′-dipyridyl) and nutrient hunger (10% M7H9 medium). Three Mtb strains have been studied: two medical isolates (drug-susceptible Beijing (BJ) and multidrug-resistant Beijing (MDR-BJ) strains) and the reference laboratory pressure, H37Rv. We investigated the proteomics profiles of those strains cultured in hectic situations after which validated the findings by transcriptional evaluation. NarJ (respiratory nitrate reductase delta chain) was considerably up-regulated on the protein degree and the mRNA degree in all three Mtb strains.

The narJ gene is a member of the narGHJI operon encoding all nitrate reductase subunits, which play a job in nitrate metabolism in the course of the adaptation of Mtb to hectic intracellular environments and the following institution of latent TB. The identification of up-regulated mRNAs and proteins of Mtb beneath stress situations might help growth of biomarkers, drug targets and vaccine antigens. The existence of latent tuberculosis an infection (LTBI) is without doubt one of the important obstacles hindering eradication of tuberculosis (TB).

Facile Preparation of Peptides for Mass Spectrometry Analysis in Bottom-Up Proteomics Workflows

 

Mass spectrometry (MS) is routinely used to establish, characterize, and quantify organic molecules. For protein evaluation, MS-based workflows might be broadly categorized as top-down or bottom-up, relying on whether or not the proteins are analyzed as intact molecules or first digested into peptides. This article outlines steps for making ready peptide samples for MS as a part of a bottom-up proteomics workflow, offering versatile strategies appropriate for discovery and focused analyses in qualitative and quantitative workflows.

Resulting samples include peptides of appropriate dimension for evaluation by MS instrumentation typically out there to trendy analysis laboratories, together with MS coupled to both liquid chromatography (LC) or matrix-assisted laser desorption/ionization (MALDI) interfaces. This article incorporates latest developments in methodologies and consumables to facilitate pattern preparation. The protocols are well-suited to customers with out prior expertise in proteomics and embody strategies for universally relevant suspension entice processing and for alternate in-solution processing to accommodate a variety of pattern varieties.

Cleanup, quantification, and fractionation procedures are additionally described. © 2021 The Authors. Basic Protocol: Preparation of high-complexity peptide samples for mass spectrometry evaluation utilizing S-Trap™ processing Alternate Protocol 1: Preparation of low- to moderate-complexity peptide samples for mass spectrometry evaluation utilizing in-solution processing Alternate Protocol 2: Detergent, polymer, and salt elimination from peptide samples earlier than mass spectrometry evaluation utilizing SP2 processing Support Protocol 1: Protein quantification utilizing Pierce 660 nm assay Support Protocol 2: Peptide quantification utilizing Pierce quantitative fluorometric peptide assay Support Protocol 3: High-pH fractionation of complicated peptide samples.

Mesh Fragmentation Improves Dissociation Efficiency in Top-down Proteomics

Discovery of Protein Modifications Using Differential Tandem Mass Spectrometry Proteomics

 

Recent research have revealed various amino acid, post-translational, and noncanonical modifications of proteins in various organisms and tissues. However, their unbiased detection and evaluation stay hindered by technical limitations. Here, we current a spectral alignment technique for the identification of protein modifications utilizing high-resolution mass spectrometry proteomics. Termed SAMPEI for spectral alignment-based modified peptide identification, this open-source algorithm is designed for the invention of practical protein and peptide signaling modifications, with out prior data of their identities.

Using artificial requirements and managed chemical labeling experiments, we exhibit its excessive specificity and sensitivity for the invention of substoichiometric protein modifications in complicated mobile extracts. SAMPEI mapping of mouse macrophage differentiation revealed various post-translational protein modifications, together with distinct types of cysteine itaconatylation. SAMPEI’s sturdy parametrization and flexibility are anticipated to facilitate the invention of organic modifications of various macromolecules.

The odor compound from Periploca sepium Bunge, 2-hydroxy-4-methoxy-benzaldehyde (HMB), is an allelochemical agent and is without doubt one of the least investigated isomers of vanillin. In this examine, we used label-free quantitative proteomics evaluation know-how to analyze the impact of HMB on the protein expression of Humulus scandens (Lour.) Merr. leaves in July 2019 on Guiyang.  A complete of 269 proteins of 624 recognized proteins have been differentially expressed, amongst which 21.18% of the proteins have been up-regulated and 32.71% down-regulated.

HemoVoid™ LC-MS On-Bead For Erythrocytes Proteomics
HVB-MS10 10 preps
EUR 521
Description: Hemoglobin Removal Kit
AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE
AGR-MS-1001 1/pk
EUR 2038
Description: Bioscience Mol Bio; Agarose
LC-LC(+)-Biotin
abx184150-5g 5 g
EUR 996
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Human Osteonectin (ON) ELISA Kit
DLR-ON-Hu-48T 48T
EUR 479
  • Should the Human Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Osteonectin (ON) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Osteonectin (ON) ELISA Kit
DLR-ON-Hu-96T 96T
EUR 621
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Description: A sandwich quantitative ELISA assay kit for detection of Human Osteonectin (ON) in samples from serum, plasma, tissue homogenates or other biological fluids.
Mouse Osteonectin (ON) ELISA Kit
DLR-ON-Mu-48T 48T
EUR 489
  • Should the Mouse Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Osteonectin (ON) ELISA Kit
DLR-ON-Mu-96T 96T
EUR 635
  • Should the Mouse Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Osteonectin (ON) ELISA Kit
DLR-ON-Ra-48T 48T
EUR 508
  • Should the Rat Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Osteonectin (ON) ELISA Kit
DLR-ON-Ra-96T 96T
EUR 661
  • Should the Rat Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Osteonectin (ON) ELISA Kit
RDR-ON-Hu-48Tests 48 Tests
EUR 500
Human Osteonectin (ON) ELISA Kit
RDR-ON-Hu-96Tests 96 Tests
EUR 692
Mouse Osteonectin (ON) ELISA Kit
RDR-ON-Mu-48Tests 48 Tests
EUR 511
Mouse Osteonectin (ON) ELISA Kit
RDR-ON-Mu-96Tests 96 Tests
EUR 709
Rat Osteonectin (ON) ELISA Kit
RDR-ON-Ra-48Tests 48 Tests
EUR 534
Rat Osteonectin (ON) ELISA Kit
RDR-ON-Ra-96Tests 96 Tests
EUR 742
Human Osteonectin (ON) ELISA Kit
RD-ON-Hu-48Tests 48 Tests
EUR 478
Human Osteonectin (ON) ELISA Kit
RD-ON-Hu-96Tests 96 Tests
EUR 662
Mouse Osteonectin (ON) ELISA Kit
RD-ON-Mu-48Tests 48 Tests
EUR 489
Mouse Osteonectin (ON) ELISA Kit
RD-ON-Mu-96Tests 96 Tests
EUR 677
Rat Osteonectin (ON) ELISA Kit
RD-ON-Ra-48Tests 48 Tests
EUR 511
Rat Osteonectin (ON) ELISA Kit
RD-ON-Ra-96Tests 96 Tests
EUR 709
AlbuVoid™ Kit
AVK-05 5 preps
EUR 405
Description: Albumin Removal Kit
AlbuVoid™ Kit
AVK-10 10 preps
EUR 552
Description: Albumin Removal Kit
AlbuVoid™ Kit
AVK-50 50 preps
EUR 1547
Description: Albumin Removal Kit
Trypsin, Recombinant, Proteomics grade
P1228-1000
EUR 196
Trypsin, Recombinant, Proteomics grade
P1228-10000
EUR 1224
Trypsin, Recombinant, Proteomics grade
P1228-5000
EUR 615
Biotin-LC-LC-NHS
2346-1000
EUR 1675
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2346-250
EUR 588
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2346-50
EUR 196
Biotin-LC-LC-NHS
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Description: Albuvoid™ Buffer
AlbuVoid Binding Buffer AVBB
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EUR 245
Description: Albuvoid™ Buffer
AlbuVoid Buffer Kit - 1000
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EUR 1166
Description: Albuvoid™ Buffer
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AVBK-500 500 mL
EUR 615
Description: Albuvoid™ Buffer
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Description: Albuvoid™ Buffer
AlbuVoid Elution Buffer AVEB
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EUR 245
Description: Albuvoid™ Buffer
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Description: Albuvoid™ Buffer
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Description: Albuvoid™ Buffer
AlbuVoid™ PLUS Kit
NP-AVK-05 5 preps
EUR 405
Description: Albumin Removal Kit
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NP-AVK10 10 preps
EUR 587
Description: Albumin Removal Kit
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (3 biotin/BSA)
7097-25
EUR 222
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (3 biotin/BSA)
7097-5
EUR 115
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7098-25
EUR 233
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (5 biotin/BSA)
7098-5
EUR 120
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (12 biotin/BSA)
7099-25
EUR 244
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (12 biotin/BSA)
7099-5
EUR 131
101Bio Protein Solubilization Reagent for MS
P5W11 NULL
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Micro Bead Sterlizer
B1201-E 1 PC
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B1202-E 1 PC
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MS-1020
1911-1
EUR 164
Ms-275
20-abx182582
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MS 275
abx076834-1mg 1 mg
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GL5031-1MG 1 mg
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B1201-BEAD 1 PC
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LC-Protein Kinase G Substrate; LC-Gsubtide (Biotin)
20-abx265657
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E-EL-H1726 1 plate of 96 wells
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  • Gentaur's MS ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human MS. During the reaction, Human MS in the sample or standard competes with a fixed amount of Human MS on the sol
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Description: A competitive ELISA kit for quantitative measurement of Human MS (Melatonin Sulfate) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human Melatonin sulfate (MS)
KTE61523-48T 48T
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Description: Quantitative sandwich ELISA for measuring Human Melatonin sulfate (MS) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Melatonin sulfate (MS)
KTE61523-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Human Melatonin sulfate (MS) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Melatonin sulfate (MS)
KTE61523-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Human Melatonin sulfate (MS) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Kininogen LC antibody
23031-100ul 100ul
EUR 390
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EUR 436
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EUR 142
Biotin-LC-Kemptide
5-00800 4 x 1mg Ask for price
CD49f LC antibody
70R-49925 100 ul
EUR 244
Description: Purified Polyclonal CD49f LC antibody
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70R-49932 100 ul
EUR 244
Description: Purified Polyclonal CD49e LC antibody
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20-abx009097
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sulfo-LC-(+)-Biotin
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20-abx185861
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CD224 LC Antibody
20-abx121054
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Myeloperoxidase LC Antibody
20-abx121172
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sulfo-LC-SPDP
ADC-L-133 unit Ask for price
NHS-LC-Biotin
A8004-100 100 mg
EUR 119
Description: NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end.
NHS-LC-Biotin
A8004-1000 1 g
EUR 548
Description: NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end.
Iodoacetyl-LC-Biotin
A8009-100 100 mg
EUR 264
Description: Iodoacetyl-LC-Biotin (N-iodoacetyl-N-biotinylhexylenediamine), a sulfhydryl-reactive biotinylation agent, is a water-insoluble reagent that requires the dissolution of suitable solvents, including dimethyl sulfoxide (DMSO) and dimethylformamide (DMF).
Biotin LC hydrazide
HY-101885 5mg
EUR 119
pPINK- LC Plasmid
PVT4035 2 ug
EUR 325
BrightGreen miRNA qPCR MasterMix-No Dye
MasterMix-mS 4 x 1.25 ml for 500 reactions
EUR 151
Bangs Lab Bead Solution
SOLN1-1000 1000 ML
EUR 155.06
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres
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EUR 212.7
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres
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SOLN1-500 500 ML
EUR 98.51
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres
uv lamp 2x15w, 254/365nm
VL215-LC ea
EUR 817
uv lamp 2x6w, 254/365nm
VL6-LC ea
EUR 538
darkroom 254/365nm
VLCN15-LC ea
EUR 1535
CLIA kit for Human ON (Osteonectin)
E-CL-H0889 1 plate of 96 wells
EUR 584
  • Gentaur's ON CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ON . Standards or samples are added to the micro CLIA plate wells and combined with the spec
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Description: A sandwich CLIA kit for quantitative measurement of Human ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
CLIA kit for Mouse ON (Osteonectin)
E-CL-M0530 1 plate of 96 wells
EUR 584
  • Gentaur's ON CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ON . Standards or samples are added to the micro CLIA plate wells and combined with the spec
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Description: A sandwich CLIA kit for quantitative measurement of Mouse ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
CLIA kit for Rat ON (Osteonectin)
E-CL-R0498 1 plate of 96 wells
EUR 584
  • Gentaur's ON CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ON . Standards or samples are added to the micro CLIA plate wells and combined with the specif
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Description: A sandwich CLIA kit for quantitative measurement of Rat ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human ON (Osteonectin)
E-EL-H1351 1 plate of 96 wells
EUR 534
  • Gentaur's ON ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ON. Standards or samples are added to the micro ELISA plate wells and combined with the sp
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Description: A sandwich ELISA kit for quantitative measurement of Human ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Mouse ON (Osteonectin)
E-EL-M0867 1 plate of 96 wells
EUR 534
  • Gentaur's ON ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ON. Standards or samples are added to the micro ELISA plate wells and combined with the sp
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Description: A sandwich ELISA kit for quantitative measurement of Mouse ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Rat ON (Osteonectin)
E-EL-R0701 1 plate of 96 wells
EUR 534
  • Gentaur's ON ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ON. Standards or samples are added to the micro ELISA plate wells and combined with the spec
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Description: A sandwich ELISA kit for quantitative measurement of Rat ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human ON (Osteonectin)
ELK1682 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteonectin (ON).
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Description: A sandwich ELISA kit for detection of Osteonectin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Rat ON (Osteonectin)
ELK1683 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteonectin (ON).
  • Show more
Description: A sandwich ELISA kit for detection of Osteonectin from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Mouse ON (Osteonectin)
ELK1684 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteonectin (ON).
  • Show more
Description: A sandwich ELISA kit for detection of Osteonectin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Human Osteonectin (ON)
KTE60336-48T 48T
EUR 354
  • Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone min
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Description: Quantitative sandwich ELISA for measuring Human Osteonectin (ON) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Osteonectin (ON)
KTE60336-5platesof96wells 5 plates of 96 wells
EUR 2252
  • Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone min
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Osteonectin (ON) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Osteonectin (ON)
KTE60336-96T 96T
EUR 572
  • Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone min
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Osteonectin (ON) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
MS 245 oxalate
B5540-10 10 mg
EUR 292
MS 245 oxalate
B5540-5 5 mg
EUR 210
Methyl-PEG3-MS
abx186609-250mg 250 mg
EUR 439
  • Shipped within 1-2 weeks.
MS-275 (Entinostat)
E1KS1053 25 mg
EUR 247
Biotin-LC-Protein Kinase G Substrate; Biotin-LC-G-Subtide
5-00802 4 x 5mg Ask for price
Sulfo-NHS-LC-Biotin
2326-100
EUR 311
Sulfo-NHS-LC-Biotin
2326-50
EUR 202
Sulfo-NHS-LC-Biotin
2326-set
EUR 213
Polyclonal CD49e LC Antibody
APR05533G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CD49e LC . This antibody is tested and proven to work in the following applications:
Complement C1R LC antibody
70R-49435 100 ul
EUR 244
Description: Purified Polyclonal Complement C1R LC antibody
Cathepsin D LC antibody
70R-49618 100 ul
EUR 244
Description: Purified Polyclonal Cathepsin D LC antibody
Cathepsin D LC Antibody
20-abx009376
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
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  • 30 ul
  • Shipped within 5-10 working days.
Complement C1R LC Antibody
20-abx009532
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
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  • 30 ul
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Cathepsin C LC Antibody
20-abx121043
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
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CD41 LC 1 Antibody
20-abx121061
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
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  • 30 ul
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Factor IX LC Antibody
20-abx121094
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
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Factor XI LC Antibody
20-abx121097
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
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  • 30 ul
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CD224 LC Blocking Peptide
20-abx161054
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
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Enteropeptidase LC Blocking Peptide
20-abx161089
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
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MASP1 LC Blocking Peptide
20-abx161154
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
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Myeloperoxidase LC Blocking Peptide
20-abx161172
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
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CD49f LC Blocking Peptide
20-abx062800
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
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CD49e LC Blocking Peptide
20-abx062807
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
LC-Kemptide (Biotin) Peptide
20-abx265999
  • EUR 384.00
  • EUR 592.00
  • EUR 300.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.
Sulfo-NHS-LC-Biotin
A8003-100 100 mg
EUR 142
Description: Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent.
Sulfo-NHS-LC-Biotin
A8003-1000 1 g
EUR 572
Description: Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent.
ExoFACS? Kit for Serum Exosomes
K1233-20
EUR 794
AffiPure Ni-NTA Agarose Bead,For His-Tagged Protein Purification
N3505-010 10ml
EUR 317
AffiPure Ni-NTA Agarose Bead,For His-Tagged Protein Purification
N3505-050 50ml
EUR 833
ON/ Rat ON ELISA Kit
ELA-E0791r 96 Tests
EUR 886
DEAE Rapid Run Agarose Bead
DEAERR-25 25 ml
EUR 102
DEAE Rapid Run Agarose Bead
DEAERR-300 300 ml
EUR 492
CM Rapid Run Agarose Bead
CMRR-25 25 ml
EUR 102
CM Rapid Run Agarose Bead
CMRR-300 300 ml
EUR 492
Bead Sample Pack - 50mL Set
BSP-50B2 1pack
EUR 404
Description: Bead sample pack for Bullet Blender 50mL tube models. Includes 20mL of: ZROB05, ZROB20, SSB02, SSB14B, SSB48, SSB60, SSUFO35 and SSUFO56.
Bead Sample Pack - 5E Set
BSP-5E 1pack
EUR 252
Description: Bead sample pack for Bullet Blender 5E models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB48, SSUFO35 and SSUFO56.
Bead Sample Pack - 5M Set
BSP-5Y 1pack
EUR 241
Description: Bead sample pack for Bullet Blender 5mL Storm models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB60, SSB110 and SSUFO35.
Bead Sample Pack - Full Set
BSP-ALL2 1pack
EUR 384
Description: Bead sample pack inlcudes sampling of all 20 bead types in 10mL tubes.
Bead Sample Pack - Cell Cultures
BSP-CC2 1pack
EUR 195
Description: Bead sample pack for homogenization of cell cultures. Includes 10mL of: GB01, GB05, ZROB015, ZROB05 and SSB02.
Bead Sample Pack - Microcentrifuge Set
BSP-MC2 1pack
EUR 232
Description: Bead sample pack for Bullet Blender 1.5mL tube models. Includes 10mL of: ZROB015, ZROB05, ZROB10, ZROB20, SSB02, SSB14B, SSB32 and SSUFO35.
Bead Sample Pack - Organ Tissues
BSP-OT3 1pack
EUR 235
Description: Bead sample pack for homogenization of organ tissues. Includes 10mL of: ZROB05, ZROB10, ZROB20, SSB14B, SSB32 and SSUFO35.
SP Rapid Run Agarose Bead
SPRR-25 25 ml
EUR 102
SP Rapid Run Agarose Bead
SPRR-300 300 ml
EUR 492
Anti-Ms Blnk Purified
11-411-C025 0.025 mg
EUR 122
Anti-Ms Blnk Purified
11-411-C100 0.1 mg
EUR 204
Anti-Ms CD45 Purified
11-553-C100 0.1 mg
EUR 113
Anti-Ms CD9 Purified
11-567-C100 0.1 mg
EUR 122
Anti-Ms Bim Purified
11-569-C025 0.025 mg
EUR 149
Anti-Ms Bim Purified
11-569-C100 0.1 mg
EUR 258
Anti-Ms CD3 Purified
11-578-C025 0.025 mg
EUR 76

These proteins have been categorized into 11 cell elements and greater than 20% of differentially expressed proteins have been situated in cell membrane and chloroplast. Functional classification evaluation confirmed that 12 molecular capabilities have been altered upon HMB therapy, and the ratio of catalytic exercise was the very best (19.53%). At least 12 organic capabilities have been affected, which concerned small molecule metabolic processes, natural substance metabolic processes, gene expression, and photosynthesis. Our knowledge present assets and insights into the biochemical mechanism by which HMB kills weeds.